of Aqueous Extract of Nutmeg on Hyperglycaemia,
Hyperlipidaemia and Cardiac Histology Associated with
Isoproterenol-induced Myocardial Infarction in Rats
Mohammed Abdul Kareem*,
Gadhamsetty Saayi Krushna, Shaik Althaf Hussain and
Kodidhela Lakshmi Devi
of Biochemistry, S.K. University, Anantapur, Andhra
author: E-mail: firstname.lastname@example.org
Received: 14 November
Revised accepted: 18 April 2009
Journal of Pharmaceutical Research, August 2009; 8(4):
The present study was designed to evaluate the
hypoglycaemic and hypolipidaemic activities of the
aqueous extract of nutmeg (i.e., the kernel of Myristica
fragrans) in rat models against myocardial infarction
(MI) induced by isoproterenol (ISO).
pretreated with nutmeg extract (NM) at an oral dose of
100 mg/kg/day for a period of 30 days, followed by the
induction of MI by subcutaneous administration of ISO
(85 mg/kg) for two consecutive days. The heart tissue
was excised immediately, washed with chilled isotonic
saline and used in histopathological studies. Blood was
also collected from the animals and the plasma separated
was subjected to biochemical analysis.
In ISO-administered group, a significant (p < 0.05)
increase in the levels of blood glucose, plasma lipids
and lipoprotein lipase activity was observed along with
hyalinization of muscle fibres, compared NM-pretreated
ISO-administered rats. In rats treated with NM,
biochemical parameters were near normal. Histological
studies revealed reduced damage of heart tissue in
ISO-administered rats that were pretreated with NM.
NM possesses protected rats against hyperglycaemia,
hyperlipidaemia and cardiac tissue damage following MI.
Therefore, NM should be further investigated as a
prophylactic against the risk of MI.
Isoproterenol; Myocardial infarction; Glucose; Lipids;
Lipoprotein lipase; Nutmeg; Myristica fragrans.