Investigation of the Leaves and Stems of Viburnum
erubescens Wall.ex DC
K Prabhu*1, PK Karar1, K
Ponnudurai1 and S Hemalatha2
of Pharmacognosy, Nandini Nagar Mahavidyalaya College of
Pharmacy, Nawabganj – 271303, Gonda, 2Department
of Pharmaceutics, IT-Banaras Hindu University, Varanasi
- 221005, Uttar Pradesh, India.
+91-9935745590, Fax: +91-5260-241444
Received: 8 May 2009
Revised accepted: 20 September 2009
Journal of Pharmaceutical Research, December 2009; 8(6):
investigations were carried out on the leaves and stems
of Viburnum erubescens Wall.ex DC to record
parameters for identifying and differentiating various
species of Viburnum.
The research specimens
were authenticated and preserved both in fresh and dry
forms. The leaves and stems were morphologically
screened followed by anatomical studies with the aid of
Labphot 2 microscopic units. Powder microscopy and
micrometric studies, including leaf constants, were
performed using suitable tools and reagents under
different magnifications. Physicochemical parameters
such as alcohol and aqueous extractive values, ash
values, crude fibre content for stem, and fluorescence
properties were also determined. The specimens were
subjected to successive extraction processes by Soxhlet
method using solvents of increasing polarity.
Qualitative chemical screening tests with suitable
reagents were also undertaken.
The leaves were petiolate
and opposite, acuminate apex with unequal base, abaxial
surfaces which turned blackish on storage, and had
irritant smell with a slightly bitter taste. The vein
islets were squarish and polygonal. Trichomes and
club-shaped glandular trichomes were evident. The barks
were hard and brittle with abundant fissures. The
periderm was four-layered followed by homogenous
parenchyma. The phloem region appeared with sclerenchyma
elements; libriform fibres and tanniferous cells were
also evident. The presence of phytosterols,
triterpenoids, glycosides (saponins) and phenolic
compounds (flavonoids and procyanidins) was positive for
both the leaf and the stem.
The findings of this study
could be useful in identifying Viburnum species, both in
whole and powder form. Also, the parameters derived from
this study may be useful in differentiating this species
from the rest of its relatives.
investigation; Amoeboid; Caprifoliaceae;
Libriform; Stone cells; Viburnum erubescens.